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KMID : 0603820160220020029
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Journal of Experimental & Biomedical Science
2016 Volume.22 No. 2 p.29 ~ p.36
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Development of a Novel Immunochromatographic Assay for Rapid Detection of OXA-23 β-lactamase-producing Acinetobacter baumannii
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Ji Gil-Young
Song Hyung-Geun
Jo Mi-Young
Hong Seung-Bok
Shin Kyeong-Seob
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Abstract
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Among the several agents causing carbapenem resistance of Acinetobacter baumannii, the most common cause is OXA-23 β-lactamase, which is known to hydrolyze carbapenem. To effectively control dissemination of carbapenem-resistant Acinetobacter baumannii (CRAB), development of both rapid and easy-to-use detection methods are required. The aim of this study is to develop a novel immunochromatographic assay (ICA) for rapid detection of OXA-23 β-lactamase. Of the seven monoclonal antibodies (mAbs) screened by ELISA, four mAbs (4G6, 4H6, 6G4, 9A4) exhibited high reactivity. Of these four specific antibodies, the combination of 6G4/4G6 showed the greatest reactivity and this combination of mAbs (6G4/4G6 mAbs) was used to develop the OXA-23 ${\beta}$ 수식 이미지-lactamase ICA. Of 102 A. baumannii isolates tested, the OXA-23 β-lactamase ICA results were consistent with PCR analysis except one false positive and one false negative isolate. The overall sensitivity and specificity were 98.36% and 97.56%, respectively. In conclusion, to the best of our knowledge, we have developed the first specific antibody set to detect OXA-23 β-lactamase using an ICA kit. This novel ICA can be used as a reliable and easy-to-use immunological assay for detection of OXA-23 β-lactamase producing CRAB in clinical laboratories.
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KEYWORD
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Acinetobacter baumannii , OXA-23 β-lactamase , Immunochromatographic assay , Rapid detection
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